Directions
Directions:A) Enter the peptide in FASTA format.
>header
LLKKLLRRLRKLKKLGGR (peptide)
B) All headers will be truncated to 15 characters in length and all large peptides will be truncated to 150 amino acids.
C) There is no limit to the number of sequences in AGRAMP.
D) Choose “9letter” or “charge” from the Alphabet pull-down menu
E) Choose “3gram” or “2gram from the Ngram pull-down menu
D) Hit submit
Generally, 9gram 3letter will be predict the best candidates. However, predictions based on a 2gram model will be faster. Many successful AMPs tend to be charged an thus searching using models based on charge might be useful. AMPs also tend to be short. However, there are many considerations to take into account.
including physiochemical properties of AMPs